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Journal of the American Podiatric Medical Association
Volume 94 Number 4 356-362 2004
Copyright © 2004 American Podiatric Medical Association

Scanning Electron Microscope Imaging of Onychomycosis

William P. Scherer, DPM, MS * and Michael David Scherer, BS {dagger}

* Private practice, Boca Raton, FL.
{dagger} College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL. (Research completed during fourth year, University of Miami, Coral Gables, FL.)

Corresponding author: Michael David Scherer, BS, PO Box 272207, Boca Raton, FL 33427 (e-mail: Michael{at}scherer.net).

Abstract

Although scanning electron microscope technology has been used for more than 60 years in many fields of medical research, no studies have focused on obtaining high-resolution microscopic images of onychomycosis of the toenail caused by Trichophyton rubrum in a geriatric population. To provide new insight into the intricate structure and behavior of chronic toenail onychomycosis, we produced three-dimensional images of onychomycosis obtained from two geriatric patients with confirmed growth of T rubrum. The photomicrographs illustrate the pervasive integration and penetration of the fungus hyphal elements, underscoring the clinical difficulty of obtaining rapid treatment of fungal infections in the distal and lateral subungual space of the human toenail. Although the scanning electron microscope may not be a practical diagnostic tool for most physicians, it remains invaluable for the researcher to obtain insight into the spatial orientation, behavior, and appearance of onychomycosis. (J Am Podiatr Med Assoc 94(4): 356–362, 2004)




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W. P. Scherer and M. D. Scherer
A Comparison of Results from Two Mycology Laboratories for the Diagnosis of Onychomycosis: A Study of 85 Cases in a Geriatric Population
J Am Podiatr Med Assoc, November 1, 2004; 94(6): 528 - 534.
[Abstract] [Full Text] [PDF]


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